At the 3rd RNA-Puzzles Meeting held at the Université du Québec à Montréal(UQAM) in Canada on September 21 and September 22, 2023, Prof. Eric Westhof initiated and concluded the event, encapsulating the salient features and challenges of Puzzle Round V, along with introducing the revamped registration and submission protocols for RNA puzzles. Day one spotlighted prediction groups showcasing their RNA 3D predictive algorithms, with notable mentions like Prof. Yaoqi Zhou, Prof. Nikolay Dokholyan, and Prof. Shi-Jie Chen. Day two centered around lectures highlighting RNA structural attributes by luminaries like Prof. Dave Mathews, Prof. Alberto Perez, and Prof. Marcin Magnus. This forum facilitated profound academic discussions and exchange with international counterparts, propelling the boundaries of RNA structural prediction.